qnrB19 gene bracketed by IS26 on a 40-kilobase IncR plasmid from an Escherichia coli isolate from a veal calf.

qnrB19 genes have been reported in Escherichia coli, Escherichia hermannii, Salmonella enterica, and Klebsiella spp., located on IncN, IncL/M (human isolates), and ColE-like (both human and chicken isolates) plasmids (2, 6, 8, 9, 11, 13, 14, 16). This study describes the characterization of the genetic environment of a plasmid-mediated qnrB19 gene identified in E. coli isolated from a veal calf in the Netherlands. E. coli strain 013.1 was selected from a fecal sample grown on a MacConkey agar plate supplemented with 0.125 mg/liter ciprofloxacin. This strain showed MIC values of 0.25 mg/liter and 16 mg/liter for ciprofloxacin and nalidixic acid, respectively, suggesting the presence of a plasmid-mediated quinolone resistance mechanism (10, 18). PCR as described for qnrA and qnrS (4), qnrB (3), qnrC (20), qnrD (5), qepA (17), and aac(6 )-Ib-cr (15) and sequence analysis revealed a qnrB19 gene (12), whereas chromosomal mutations in the topoisomerase genes gyrA and/or gyrB and parC and/or parE were absent (7). Conjugation experiments using standard broth mating experiments with rifampin-resistant E. coli K-12 as the recipient were not successful. However, DH10B (Gibco Invitrogen) transformants (designated 013.1-T) could be selected on Luria-Bertani agar plates supplemented with 0.03 mg/liter ciprofloxacin (Sigma) using plasmids isolated from strain 013.1, indicating that the qnrB19 gene was located on the plasmid. Strain 013.1-T showed MIC values for ciprofloxacin and nalidixic acid of 0.12 mg/liter and 4 mg/liter, respectively. Untransformed DH10B cells were susceptible to ciprofloxacin (MIC 0.008 mg/liter) and nalidixic acid (MIC 4 mg/liter). Strain 013.1-T did not harbor the qepA or aac(6 )-Ib-cr gene or mutations in the chromosomally located gyrA, gyrB, parC, or parE genes. Plasmid sizes were determined by S1 pulsed-field gel electrophoresis (PFGE) (19), as well as by S1 gel electrophoresis (GE) (running at 80 V for 4 h). Strain 013.1 harbored a 40-kb plasmid and a 2.6-kb plasmid. Strain 013.1-T harbored a 40-kb plasmid only. Subsequently, the plasmids were identified by PCR-based replicon typing (PBRT) (1, 9) as replicon type IncR and as ColE in strain 013.1. Transformant 013.1-T harbored only an IncR-type plasmid, designated p013.1IncR. Sequence analysis of the qnr gene and its flanking regions was performed by primer walking analysis on purified DNA of p013.1IncR. The qnrB19 gene was bracketed by two identical IS26 insertion sequences (Fig. 1A). Further downstream of the qnrB19 gene, an aphA1 gene was identified, followed by a partial IS26 sequence, which was interrupted by transposable element Tn5393 harboring a strB gene. Two regions, one comprising qnrB19, IRR2, and a IS26 sequence, and a second region comprising aphA1 and the partial sequence of IS26 (Fig. 1A and B), both showed one point mutation difference compared to the sequences published previously (6). Both fulllength IS26 elements bracketing the qnrB19 gene are identical to each other (Fig. 1A) and to the one previously described downstream of aphA1 (Fig. 1B) (6). ISEcp1C, located with qnrB19 on Tn2012 as described by Cattoir et al. (2) (Fig. 1C), was not observed in the present study.